OK, so maybe it was more than just once
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Four years earlier, two thirds of that creative team (Friedrich and Ayers, only Ayers was inking Herb Trimpe) killed Fury for the FIRST time in Fury, Agent of SHIELD #15, which was, at the time, the last issue of the title (it came back a year later).
Fury is at a concert with his girlfriend when a HYDRA agent named Bulls-Eye shoots him dead…